Primary Cell & Tumor Models

Primary Cell and Tumor Models

Primary Cell Models

Multiple human and other mammalian cell cultures have been established using CR technology.   Key advantages of CR primary cell cultures is the ability to create cultures from specific patient samples, the genetic stability of cells over extended passages, and retention of tissue-specific lineage commitment and phenotypic functionality. The video at left is of an airway cell culture expanded using EpiX™ technology and subsequently differentiated in submerged mode to create a 2D multi-lineage cell model including cells with actively beating cilia.

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Tumor Cell Models

Efforts by hundreds of researchers during the past half century have been expended on attempts to generate stable cell lines from primary tumors that can be used as in vitro models to represent their behavior in vivo. However, problems of limited lifespan, difficulty of maintaining tumor heterogeneity despite selective pressures during in vitro culture, and the need for matched pairs of normal and tumor tissue from the same patients are well recognized by cancer researchers. CR technology is now being used to create next-generation cancer models that address all of these limitations.   In addition, our technology is being employed to generate cell cultures from patient-derived xenografts, metastatic tumors and liquid biopsies to provide highly relevant cell models for basic and translational research studies in future.

A CR-generated culture of human prostate tumor cells, grown under 2D (left) and 3D (middle) conditions. The image on the right shows a solid tumor generated by subcutaneous injection of CR-expanded human prostate tumor cells into immunodeficient mice.

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