Publication Link

Publication Links

Conditional reprogrammed human limbal epithelial cells represent a novel in vitro cell model for drug responses.

Wang L, Ye L, Wei G, Chen Y, Ye L, Wu X, Zeng Z, Wang Y, Yin G, Long X, Li H.

(2018) Biochem Biophys Res Commun. May 23;499(4):735-742. PMID: 29577905

Long-term Culture and Cloning of Primary Human Bronchial Basal Cells that Maintain Multipotent Differentiation Capacity and CFTR Channel Function.

Peters-Hall JR, Coquelin ML, Torres MJ, LaRanger R, Alabi BR, Sho S, Calva-Moreno JF, Thomas PJ, Shay JW.

(2018) Am J Physiol Lung Cell Mol Physiol. May 3.  PMID: 29722564

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study.

Brewington JJ, Filbrandt ET, LaRosa FJ 3rd, Moncivaiz JD, Ostmann AJ, Strecker LM, Clancy JP.

(2018) J Vis Exp. Apr 11;(134). PMID: 29708545

Characterization of primary human mammary epithelial cells isolated and propagated by conditional reprogrammed cell culture.

Jin L, Qu Y, Gomez LJ, Chung S, Han B, Gao B, Yue Y, Gong Y, Liu X, Amersi F, Dang C, Giuliano AE, Cui X.

(2017) Oncotarget. Dec 22;9(14):11503-11514. PMID: 29545915

Patient-derived conditionally reprogrammed cells maintain intra-tumor genetic heterogeneity.

Correa BRS, Hu J, Penalva LOF, Schlegel R, Rimm DL, Galante PAF, Agarwal S.

(2018) Sci Rep. Mar 6;8(1):4097. PMID: 29511269

Expanding primary cells from mucoepidermoid and other salivary gland neoplasms for genetic and chemosensitivity testing.

Alamri AM, Liu X, Blancato JK, Haddad BR, Wang W, Zhong X, Choudhary S, Krawczyk E, Kallakury BV, Davidson BJ, Furth PA.

(2018) Dis Model Mech. Jan 29;11(1). PMID: 29419396

Characterization of the effects of defined, multidimensional culture conditions on conditionally reprogrammed primary human prostate cells.

Tricoli L, Naeem A, Parasido E, Mikhaiel JP, Choudhry MU, Berry DL, Abdelgawad IA, Lee RJ, Feldman AS, Ihemelandu C, Avantaggiati M, Kumar D, Byers S, Gallagher R, Wulfkuhle J, Petricoin E, Rodriguez O, Albanese C.

(2017) Oncotarget. Dec 18;9(2):2193-2207. PMID: 29416764

Conditionally reprogrammed primary airway epithelial cells maintain morphology, lineage and disease specific functional characteristics.

Martinovich KM, Iosifidis T, Buckley AG, Looi K, Ling KM, Sutanto EN, Kicic-Starcevich E, Garratt LW, Shaw NC, Montgomery S, Lannigan FJ, Knight DA, Kicic A, Stick SM.

(2017) Sci Rep. Dec 21;7(1):17971. PMID: 29269735

Generation of stable PDX derived cell lines using conditional reprogramming.

Borodovsky A, McQuiston TJ, Stetson D, Ahmed A, Whitston D, Zhang J, Grondine M, Lawson D, Challberg SS, Zinda M, Pollok BA, Dougherty BA, D'Cruz CM.

(2017) Mol Cancer. Dec 6;16(1):177. PMID: 29212548

Genomic comparison of early-passage conditionally reprogrammed breast cancer cells to their corresponding primary tumors.

Mahajan AS, Sugita BM, Duttargi AN, Saenz F, Krawczyk E, McCutcheon JN, Fonseca AS, Kallakury B, Pohlmann P, Gusev Y, Cavalli LR.

(2017) PLoS One. Oct 19;12(10):e0186190. PMID: 29049316

Mutation-specific downregulation of CFTR2 variants by gating potentiators.

Avramescu RG, Kai Y, Xu H, Bidaud-Meynard A, Schnúr A, Frenkiel S, Matouk E, Veit G, Lukacs GL.

(2017) Hum Mol Genet. Dec 15;26(24):4873-4885. PMID: 29040544

Conditional reprogramming of pediatric airway epithelial cells: A new human model to investigate early-life respiratory disorders.

Wolf S, Perez GF, Mukharesh L, Isaza N, Preciado D, Freishtat RJ, Pillai D, Rose MC, Nino G.

(2017) Pediatr Allergy Immunol. Dec;28(8):810-817. PMID: 28981980

Emerging Opportunities for Target Discovery in Rare Cancers.

Sharifnia T, Hong AL, Painter CA, Boehm JS.

(2017) Cell Chem Biol. Sep 21;24(9):1075-1091. Review. PMID: 28938087

A multiplex preclinical model for adenoid cystic carcinoma of the salivary gland identifies regorafenib as a potential therapeutic drug.

Chen C, Choudhury S, Wangsa D, Lescott CJ, Wilkins DJ, Sripadhan P, Liu X, Wangsa D, Ried T, Moskaluk C, Wick MJ, Glasgow E, Schlegel R, Agarwal S.

(2017) Sci Rep. Sep 12;7(1):11410. PMID: 28900283

Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells.

LaRanger R, Peters-Hall JR, Coquelin M, Alabi BR, Chen CT, Wright WE, Shay JW.

(2018) Tissue Eng Part A. Apr;24(7-8):559-568. PMID: 28726588

Expanding primary cells from mucoepidermoid and other salivary gland neoplasms for genetic and chemosensitivity testing.

Alamri AM, Liu X, Blancato JK, Haddad BR, Wang W, Zhong X, Choudhary S, Krawczyk E, Kallakury BV, Davidson BJ, Furth PA.

(2018) Dis Model Mech. ePub of Jan 29. PMID: 29419396

Conditional cell reprogramming involves non-canonical β-catenin activation and mTOR-mediated inactivation of Akt.

Suprynowicz FA, Kamonjoh CM, Krawczyk E, Agarwal S, Wellstein A, Agboke FA, Choudhury S, Liu X, Schlegel R.

(2017) PLoS One. Jul 10;12(7):e0180897. PMID: 28700668

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation.

Jensen TJ, Foster C, Sayej W, Finck CM.

(2017) J Vis Exp. Mar 22;(121). PMID: 28362412

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation.

Tricoli L, Berry DL, Albanese C.

(2017) J Vis Exp. Feb 13;(120). PMID: 28287583

Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens.

Liu X, Krawczyk E, Suprynowicz FA, Palechor-Ceron N, Yuan H, Dakic A, Simic V, Zheng YL, Sripadhan P, Chen C, Lu J, Hou TW, Choudhury S, Kallakury B, Tang DG, Darling T, Thangapazham R, Timofeeva O, Dritschilo A, Randell SH, Albanese C, Agarwal S, Schlegel R.

(2017) Nat Protoc. Feb;12(2):439-451. PMID: 28125105

Pharmacological Rescue of Conditionally Reprogrammed Cystic Fibrosis Bronchial Epithelial Cells.

Gentzsch M, Boyles SE, Cheluvaraju C, Chaudhry IG, Quinney NL, Cho C, Dang H, Liu X, Schlegel R, Randell SH.

(2017) Am J Respir Cell Mol Biol. May;56(5):568-574. PMID: 27983869

Primary cancer cell culture: mammary-optimized vs conditional reprogramming.

Alamri AM, Kang K, Groeneveld S, Wang W, Zhong X, Kallakury B, Hennighausen L, Liu X, Furth PA.

(2016) Endocr Relat Cancer. Jul;23(7):535-54. PMID: 27267121

Correctors and Potentiators Rescue Function of the Truncated W1282X-Cystic Fibrosis Transmembrane Regulator (CFTR) Translation Product.

Haggie PM, Phuan PW, Tan JA, Xu H, Avramescu RG, Perdomo D, Zlock L, Nielson DW, Finkbeiner WE, Lukacs GL, Verkman AS.

(2017) J Biol Chem. Jan 20;292(3):771-785. PMID: 27895116

Comprehensive Drug Testing of Patient-derived Conditionally Reprogrammed Cells from Castration-resistant Prostate Cancer.

Saeed K, Rahkama V, Eldfors S, Bychkov D, Mpindi JP, Yadav B, Paavolainen L, Aittokallio T, Heckman C, Wennerberg K, Peehl DM, Horvath P, Mirtti T, Rannikko A, Kallioniemi O, Östling P, Af Hällström TM.

(2017) Eur Urol. Mar;71(3):319-327. PMID: 27160946

Characterization of Rat Meibomian Gland Ion and Fluid Transport.

Yu D, Davis RM, Aita M, Burns KA, Clapp PW, Gilmore RC, Chua M, O'Neal WK, Schlegel R, Randell SH, C Boucher R.

(2016) Invest Ophthalmol Vis Sci. Apr 1;57(4):2328-43. PMID: 27127933

Rapid Expansion of Human Epithelial Stem Cells Suitable for Airway Tissue Engineering

Butler CR, Hynds RE, Gowers KHC, Lee DDH, Brown JM, Crowley C,Teixeira VH, Smith CM, Urbani L, Hamilton NJ, Thakrar RM, Booth HL, Birchall MA, De Coppi P, Giangreco A, O’Callaghan C, Janes SM

(2016) Am J Respir Crit Care Med. [Epub]. PMID: 26840431

Airway Progenitor Clone Formation is Enhanced by Y-27632-dependent Changes in the Transcriptome

Reynolds SD, Rios C, Wesolowska-Andersen A, Zhuang Y, Pinter M, Happoldt C, Hill CL, Lallier SW,Cosgrove GP, Solomon GM, Nichols DP, Seibold MA

(2016) Am J Respir Cell Mol Biol. [ePub]. PMID: 27144410

Pseudo-immortalization of postnatal cochlear progenitor cells yields a scalable cell line capable of transcriptionally regulating mature hair cell genes

Walters BJ, Diao S, Zheng F, Walters BJ, Layman WS, Zuo J

(2015) Nature Sci Rep. 2015 5:17792-17804. PMID: 26639154

CRISPR-Cas9-mediated Gene Knockout in Primary Human Airway Epithelial Cells Reveals a Proinflammatory Role for MUC18.

Chu HW, Rios C, Huang C, Wesolowska-Andersen A, Burchard EG, O'Connor BP, Fingerlin TE, Nichols D, Reynolds SD, Seibold MA

(2015) Gene Therapy 22(10):822-829. PMID: 26043872

An ecosystem of cancer cell line factories to support a cancer dependency map

Boehm JS, Golub TR

(2015) Nat Rev Genet 16: 373-374. PMID: 26077369

Patient-derived models of acquired resistance can identify effective drug combinations for cancer

Crystal AS, Shaw AT, Sequist LV, Friboulet L, Niederst MJ, Lockerman EL, Frias RL, Gainor JF, Amzallag A, Greninger P, Lee D, Kalsy A, Gomez-Caraballo M, Elamine L, Howe E, Hur W, Lifshits E, Robinson HE, Katayama R, Faber AC, Awad MM, Ramaswamy S, Mino-Kenudson M, Iafrate AJ, Benes CH, Engelman JA

(2014) Science 346(6216):1480-1486. PMID: 25394791

Conditionally reprogrammed normal and transformed mouse mammary epithelial cells display a progenitor-cell-like phenotype

Saenz FR, Ory V, AIOtaiby M, Rosenfield S, Furlong M, Cavalli LR, Johnson MD, Liu X, Schlegel R, Wellstein A, Riegel AT

PLoS ONE 9(5):e97666. PMID: 24831228

Breaking the in vitro alveolar type II cell proliferation barrier while retaining ion transport properties

Bove PF, Dang H, Cheluvaraju C, Jones LC, Liu X, O’Neal WK, Randell SH, Schlegel R, Boucher RC

(2014) Am J Resp Cell Mol 50(4):767-776. PMID: 24191670

Radiation induces diffusible feeder cell factor(s) that cooperate with ROCK inhibitor to conditionally reprogram and immortalize epithelial cells

Palechor-Ceron N, Suprynowicz FA, Upadhyay G, Dakic A, Minas T, Simic V, Johnson M, Albanese C, Schlegel R, Liu X

(2013) Am J Pathol 183(6): 1862-1870. PMID: 24096078

Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells

Suprynowicz FA, Upadhyay G, Krawczyk E, Kramer SC, Hebert JD, Liu X, Yuan H, Cheluvaraju C, Clapp PW, Boucher RC Jr, Kamonjoh CM, Randell SH, Schlegel R

(2012) P Natl Acad Sci USA 109(49):20035-20040. PMID: 23169653

Use of reprogrammed cells to identify therapy for respiratory papillomatosis

Yuan H, Myers S, Wang J, Zhou D, Woo JA, Kallakury B, Ju A, Bazylewicz M, Carter YM, Albanese C, Grant N, Shad A, Dritschilo A, Liu X, Schlegel R

(2012) New Engl J Med 367(13):1220-1227. PMID: 23013073

ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells.

Ory V, Chapman S, Yuan H, Albanese C, Kallakury B, Timofeeva OA, Nealon C, Dakic A, Simic V, Haddad BR, Rhim JS, Dritschilo A, Riegel A, McBride A, Schlegel R

(2012) Am J Pathol 180(2):599-607. PMID: 22189618

Making every cell like HeLa a giant step for cell culture

Aggaral S, Rimm DL

(2011) Am J Patho 180(2):443-445. PMID: 22192626

Developing in vitro models of human ductal carcinoma in situ from primary tissue explants

Brown DD, Dabbs DJ, Lee AV, McGuire KP, Ahrendt GM, Bhargava R, Davidson NE, Brufsky AM, Johnson RR, Oesterreich S, McAuliffe PF

(2015) Breast Cancer Res Treat 153(2):311-21. PMID: 26283301

Primary cancer cell culture: mammary-optimized vs conditional reprogramming

Alamri AM, Kang K, Groeneveld S, Wang W, Zhong X, Kallakury B, Hennighausen L, Liu X, Furth PA

(2016) Endocr Relat Cancer 23(7):535-54. PMID: 27267121

Screening of Conditionally Reprogrammed Patient-Derived Carcinoma Cells Identifies ERCC3-MYC Interactions as a Target in Pancreatic Cancer

Beglyarova N, Banina E, Zhou Y, Mukhamadeeva R, Andrianov G, Bobrov E, Lysenko E, Skobeleva N, Gabitova L, Restifo D, Pressman M, Serebriiskii IG, Hoffman JP, Paz K, Behrens D, Khazak V, Jablonski SA, Golemis EA, Weiner LM, Astsaturov I

(2016) Clin Cancer Res Treat 22(24):6153-6163. PMID: 27384421

Conditionally reprogrammed normal and primary tumor prostate epithelial cells: a novel patient-derived cell model for studies of human prostate cancer

Timofeeva OA, Palechor-Ceron N, Li G, Yuan H, Krawczyk E, Zhong X, Liu G, Upadhyay G, Dakic A, Yu S, Fang S, Choudhury S, Zhang X, Ju A, Lee MS, Dan HC, Ji Y, Hou Y, Zheng YL, Albanese C, Rhim J, Schlegel R, Dritschilo A, Liu X

(2016) Oncotarget 10.18632/oncotarget.13937. PMID: 28009986

Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens

Liu X, Krawczyk E, Suprynowicz FA, Palechor-Ceron N, Yuan H, Dakic A, Simic V, Zheng YL, Sripadhan P, Chen C, Lu J, Hou TW, Choudhury S, Kallakury B, Tang D, Darling T, Thangapazham R, Timofeeva O, Dritschilo A, Randell SH, Albanese C, Agarwal S, Schlegel R.

(2017) Nat Protoc 12(2):439-451. PMID: 28125105