Propagenix Conditioned Medium is generated from irradiated 3T3-J2 mouse fibroblast cells (Propagenix cat # PF-1100) and CRM medium (Propagenix cat # 276-101). The Conditioned Medium contains nutrients, fetal bovine serum and growth factors required to support cell growth in the absence of growth-arrested feeder cells when supplemented with Cholera toxin. Medium does not contain antibiotics.
Unit of Measure
100 mL bottle
Store medium at -20°C until needed
Thaw at 2-8°C and continue to store at 2-8°C
Once thawed, use within two weeks
Conditional Reprogramming (CR) technology is a revolutionary new approach to generating unprecedented quantities of biologically important cell types ex vivo that retain the ability to re-adopt the relevant functionality of the tissues from which the cells originated.
When mature epithelial cells are introduced into CR culture conditions, they adopt a tissue-specific progenitor phenotype and become proliferative. Primary epithelial cells from airway tissues, retina, prostate, breast, intestine, pancreas, liver biliary duct and other tissues have all proven to be induced to replicate for extended periods under CR conditions. On removal from the CR culture conditions, these cells re-adopt their original differentiation state, hence the conditional nature of the process. No genetic modification is involved in the switch between the replicative and differentiated phenotypes, and no complex re-differentiation strategy is required for the cells to return to their normal functional phenotype. The technology has also been employed successfully to propagate cells from primary tumors, metastatic tumors and patient-derived xenografts.
A: Propagation of four tissue types using CR technology. Day 5 of culture shows large and distinct colonies of epithelial cells surrounded by irradiated feeder cells. B: Continued passaging of prostate and mammary cells in CR technology highlighting expansion potential of culture compared to respective cell type commercially available mediums.
Liu X et al. (2012). ROCK Inhibitor and Feeder Cells Induce the Conditional Reprogramming of Epithelial cells. Am J Pathol. 180(2):599-607
Product Insert & Protocols
Patient-Derived Conditionally Reprogrammed Cells Maintain Intra-Tumor Genetic Heterogeneity. Correa BRS, Hu J, Penalva LOF, Schlegel R, Rimm DL, Galante PAF, Agarwal S. (2018) Sci Rep. Mar 6;8(1):4097. PMID: 29511269
Generation Of Stable PDX Derived Cell Lines Using Conditional Reprogramming. Borodovsky A, McQuiston TJ, Stetson D, Ahmed A, Whitston D, Zhang J, Grondine M, Lawson D, Challberg SS, Zinda M, Pollok BA, Dougherty BA, D'Cruz CM. (2017) Mol Cancer. Dec 6;16(1):177. PMID: 29212548
Conditional Reprogramming And Long-Term Expansion Of Normal And Tumor Cells From Human Biospecimens. Liu X, Krawczyk E, Suprynowicz FA, Palechor-Ceron N, Yuan H, Dakic A, Simic V, Zheng YL, Sripadhan P, Chen C, Lu J, Hou TW, Choudhury S, Kallakury B, Tang DG, Darling T, Thangapazham R, Timofeeva O, Dritschilo A, Randell SH, Albanese C, Agarwal S, Schlegel R. (2017) Nat Protoc. Feb;12(2):439-451. PMID: 28125105
Conditionally Reprogrammed Cells Represent A Stem-Like State Of Adult Epithelial Cells. Suprynowicz FA, Upadhyay G, Krawczyk E, Kramer SC, Hebert JD, Liu X, Yuan H, Cheluvaraju C, Clapp PW, Boucher RC Jr, Kamonjoh CM, Randell SH, Schlegel R (2012) P Natl Acad Sci USA 109(49):20035-20040. PMID: 23169653
Airway Progenitor Clone Formation Is Enhanced By Y-27632-Dependent Changes In The Transcriptome. Reynolds SD, Rios C, Wesolowska-Andersen A, Zhuang Y, Pinter M, Happoldt C, Hill CL, Lallier SW,Cosgrove GP, Solomon GM, Nichols DP, Seibold MA (2016) Am J Respir Cell Mol Biol. [ePub]. PMID: 27144410
Radiation Induces Diffusible Feeder Cell Factor(S) That Cooperate With ROCK Inhibitor To Conditionally Reprogram And Immortalize Epithelial Cells. Palechor-Ceron N, Suprynowicz FA, Upadhyay G, Dakic A, Minas T, Simic V, Johnson M, Albanese C, Schlegel R, Liu X (2013) Am J Pathol 183(6): 1862-1870. PMID: 24096078
ROCK Inhibitor And Feeder Cells Induce The Conditional Reprogramming Of Epithelial Cells. Ory V, Chapman S, Yuan H, Albanese C, Kallakury B, Timofeeva OA, Nealon C, Dakic A, Simic V, Haddad BR, Rhim JS, Dritschilo A, Riegel A, McBride A, Schlegel R(2012) Am J Pathol 180(2):599-607. PMID: 22189618